Journal Published Online: 01 January 2005
Volume 50, Issue 1

H NMR Analysis of GHB and GBL: Further Findings on the Interconversion and a Preliminary Report on the Analysis of GHB in Serum and Urine

CODEN: JFSCAS

Abstract

A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH.

Author Information

Del Signore, AG
College of Pharmacy, University of Rhode Island, Kingston, RI
McGregor, M
University of Rhode Island, Kingston, RI
Cho, BP
College of Pharmacy, University of Rhode Island, Kingston, RI
Pages: 6
Price: $25.00
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Stock #: JFS2004179
ISSN: 0022-1198
DOI: 10.1520/JFS2004179