Blood specimens were collected from unrelated volunteer donors. DNA was extracted from blood specimens using Chelex-100 (1). DNA typing was carried out by PCR. The components of PCR were: target DNA 20 ng, primer 0.2 µmol/L, dNTPs 200 µmol/L, KCl 50 µmol/L, Tris-HCl (pH 8.3) 10 mmol/L, MgCl2 1.5 mmol/L, Taq 1U. Primer sequences: D21S1435: 5'-CCC TCT CAA TTG TTT GTC TAC C-3', 5'-ATG GCA CTG AAA TCT CTT GC-3'; D21S2055: 5'-AAC AGA ACC AAT AGG CTA TCT ATC-3', 5'-TCT CCT ACC AAG TGA TTT ACT GTA-3'.PCR conditions: D21S1435: start at 94°C for 3 min, 30 cycles consist of 35s at 94°C, 45s at 61°C, 55s at 72°C followed by a 5 min extension at 72°C. D2152055: start at 94°C for 3 min, 30 cycles consist of 35 s at 94°C, 45 s at 61°C, 55 s at 72°C followed by a 5 min extension at 72°C. The amplified products were electrophoresed in 6% polyacrylamide gel by using 100 bp ladder and allelic markers for both D21S1435 and D21S2055 as size marker, followed by sliver staining. Data were analyzed by The Promega Software, POWERSTATS. Calculating of Chi-square test was carried out for Hardy-Weinberg equilibrium test.
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