Journal Published Online: 01 January 1996
Volume 41, Issue 1

HUMTH01 Validation Studies: Effect of Substrate, Environment, and Mixtures

CODEN: JFSCAS

Abstract

New DNA typing methods need to be thoroughly validated prior to use in forensic investigations. This includes determining the effects different sample conditions have on the typeability of those samples. Biological samples routinely encountered in forensic case work were exposed to a series of different substrates, environmental conditions, and mixtures and typed for the STR HUMTH01 using PCR. None of the conditions resulted in a false typing or preferential allele amplification. It is demonstrated that the application of HUMTH01 typing methods in forensic case work can be reliable, robust, and efficient.

Author Information

van Oorschot, RAH
Victoria Forensic Science Centre (VFSC) Forensic Drive, Macleod, Victoria, Australia
Gutowski, SJ
Victoria Forensic Science Centre (VFSC) Forensic Drive, Macleod, Victoria, Australia
Robinson, SL
Victoria Forensic Science Centre (VFSC) Forensic Drive, Macleod, Victoria, Australia
Hedley, JA
Victoria Forensic Science Centre (VFSC) Forensic Drive, Macleod, Victoria, Australia
Andrew, IR
Victoria Forensic Science Centre (VFSC) Forensic Drive, Macleod, Victoria, Australia
Pages: 4
Price: $25.00
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Stock #: JFS13912J
ISSN: 0022-1198
DOI: 10.1520/JFS13912J