Standard Historical Last Updated: Aug 16, 2017
ASTM E1533-00

Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4'-6-Diamidino-2-2 Phenylindole (DAPI) Staining


1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining.

1.2 This practice does not cover direct methods for the detection of mycoplasma or other indirect methods such as enzymatical detection or DNA probes.

1.3 This practice does not cover methods for the identification of mycoplasma organisms.

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Book of Standards Volume: 11.06
Developed by Subcommittee: E48.02
Pages: 2
DOI: 10.1520/E1533-00
ICS Code: 07.100.10