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    ASTM D7464 - 20

    Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

    Active Standard ASTM D7464 | Developed by Subcommittee: D02.14

    Book of Standards Volume: 05.04

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    Significance and Use

    5.1 Representative samples of fuel products and associated substances are required for the determination of microbial contamination in fuels and fuel systems in order to accurately assess the biodeterioration risk posed to the fuel, fuel-system components or both. Uncontrolled microbial contamination can affect fuel specification properties adversely.6 As discussed in Guide D6469, microbes can cause a variety of operational problems, including filter plugging and microbially influenced corrosion (MIC), the latter of which causes valve failure, tank and pipeline failure.

    5.2 These practices for microbiological sampling decrease the risk of contaminating samples with extraneous microbes, thereby increasing the probability that the original microbial population in the sample does not change significantly between the time of sampling and the time of testing.

    5.3 The objective of sampling for microbiological testing is to obtain representative samples that are likely to reflect the degree and nature of microbial contamination in the system from which the samples are collected. Manual 477 addresses the rationale for and design of microbial contamination programs. Recognizing that microbiological contamination is not distributed uniformly throughout fuel systems, both the number and types of samples collected will normally be different from the samples collected per Practice D4057 in order to determine whether product meets specifications.

    5.4 The physical, chemical and microbiological property tests to be performed on a sample will dictate the sampling procedures, the sample quantity required, and many of the sample handling requirements.

    5.5 Fuel systems are not normally designed to facilitate optimal microbiological sampling. Consequently, the selection of sampling device and sample source reflect compromises between accessibility and suitability for meeting the sample collection objective.

    5.6 The guidance provided in Practice D4057 generally applies to this practice as well. Consequently, this practice will address only those procedures that apply uniquely to microbiological sampling.

    1. Scope

    1.1 This practice covers aspects of sample device preparation and sample handling that prevent samples from becoming contaminated with microorganisms not originally contained within the sample.

    1.2 This practice also covers sample handling considerations that reflect the perishability of samples collected for microbiological testing.

    1.3 This practice supplements Practice D4057 by providing guidance specific to the manual sampling of fuels when samples are to be tested for microbial contamination.

    1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    Petroleum Equipment Institute (PEI) Standard


    ASTM Standards

    D396 Specification for Fuel Oils

    D910 Specification for Leaded Aviation Gasolines

    D975 Specification for Diesel Fuel

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D1655 Specification for Aviation Turbine Fuels

    D2069 Specification for Marine Fuels

    D2880 Specification for Gas Turbine Fuel Oils

    D3508 Method for Evaluating Water Testing Membrane Filters for Fecal Coliform Recovery

    D3699 Specification for Kerosine

    D4057 Practice for Manual Sampling of Petroleum and Petroleum Products

    D4814 Specification for Automotive Spark-Ignition Engine Fuel

    D5245 Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses

    D6227 Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component

    D6469 Guide for Microbial Contamination in Fuels and Fuel Systems

    D6751 Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels

    D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid FuelsFiltration and Culture Procedures

    ICS Code

    ICS Number Code 07.100.99 (Other standards related to microbiology); 75.160.20 (Liquid fuels)

    UNSPSC Code

    UNSPSC Code

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D7464-20

    Citation Format

    ASTM D7464-20, Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing, ASTM International, West Conshohocken, PA, 2020, www.astm.org

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