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    ASTM D6469 - 20

    Standard Guide for Microbial Contamination in Fuels and Fuel Systems

    Active Standard ASTM D6469 | Developed by Subcommittee: D02.14

    Book of Standards Volume: 05.03

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    Significance and Use

    5.1 This guide provides information addressing the conditions that lead to fuel microbial contamination and biodegradation and the general characteristics of and strategies for controlling microbial contamination. It compliments and amplifies information provided in Practice D4418 on handling gas-turbine fuels. More detailed information may be found in Guidelines for the Investigation of Microbial Content of Liquid Fuels and for the Implementation of Avoidance and Remedial Strategies, 3rd Ed.,10 ASTM Manual 47, and Passman, 2019.11

    5.2 This guide focuses on microbial contamination in refined petroleum products and product handling systems. Uncontrolled microbial contamination in fuels and fuel systems remains a largely unrecognized but costly problem at all stages of the petroleum industry from crude oil production through fleet operations and consumer use. This guide introduces the fundamental concepts of fuel microbiology and biodeterioration control.

    5.3 This guide provides personnel who are responsible for fuel and fuel system stewardship with the background necessary to make informed decisions regarding the possible economic or safety, or both, impact of microbial contamination in their products or systems.

    1. Scope

    1.1 This guide provides personnel who have a limited microbiological background with an understanding of the symptoms, occurrence, and consequences of chronic microbial contamination. The guide also suggests means for detection and control of microbial contamination in fuels and fuel systems. This guide applies primarily to gasoline, aviation, boiler, industrial gas turbine, diesel, marine, furnace fuels and blend stocks (see Specifications D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, and D6751), and fuel systems. However, the principles discussed herein also apply generally to crude oil and all liquid petroleum fuels. ASTM Manual 472 provides a more detailed treatment of the concepts introduced in this guide; it also provides a compilation of all of the standards referenced herein that are not found in the Annual Book of ASTM Standards, Section Five on Petroleum Products and Lubricants.

    1.2 This guide is not a compilation of all of the concepts and terminology used by microbiologists, but it does provide a general understanding of microbial fuel contamination.

    1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D130 Test Method for Corrosiveness to Copper from Petroleum Products by Copper Strip Test

    D396 Specification for Fuel Oils

    D445 Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity)

    D515 Test Method for Phosphorus In Water

    D664 Test Method for Acid Number of Petroleum Products by Potentiometric Titration

    D888 Test Methods for Dissolved Oxygen in Water

    D910 Specification for Leaded Aviation Gasolines

    D974 Test Method for Acid and Base Number by Color-Indicator Titration

    D975 Specification for Diesel Fuel

    D1067 Test Methods for Acidity or Alkalinity of Water

    D1126 Test Method for Hardness in Water

    D1293 Test Methods for pH of Water

    D1298 Test Method for Density, Relative Density, or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method

    D1331 Test Methods for Surface and Interfacial Tension of Solutions of Paints, Solvents, Solutions of Surface-Active Agents, and Related Materials

    D1426 Test Methods for Ammonia Nitrogen In Water

    D1655 Specification for Aviation Turbine Fuels

    D1744 Test Method for Determination of Water in Liquid Petroleum Products by Karl Fischer Reagent

    D1976 Test Method for Elements in Water by Inductively-Coupled Plasma Atomic Emission Spectroscopy

    D2068 Test Method for Determining Filter Blocking Tendency

    D2069 Specification for Marine Fuels

    D2274 Test Method for Oxidation Stability of Distillate Fuel Oil (Accelerated Method)

    D2276 Test Method for Particulate Contaminant in Aviation Fuel by Line Sampling

    D2880 Specification for Gas Turbine Fuel Oils

    D3240 Test Method for Undissolved Water In Aviation Turbine Fuels

    D3241 Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels

    D3242 Test Method for Acidity in Aviation Turbine Fuel

    D3325 Practice for Preservation of Waterborne Oil Samples

    D3326 Practice for Preparation of Samples for Identification of Waterborne Oils

    D3328 Test Methods for Comparison of Waterborne Petroleum Oils by Gas Chromatography

    D3414 Test Method for Comparison of Waterborne Petroleum Oils by Infrared Spectroscopy

    D3699 Specification for Kerosine

    D3867 Test Methods for Nitrite-Nitrate in Water

    D3870 Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology

    D4012 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

    D4057 Practice for Manual Sampling of Petroleum and Petroleum Products

    D4176 Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)

    D4412 Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits

    D4418 Practice for Receipt, Storage, and Handling of Fuels for Gas Turbines

    D4454 Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy

    D4814 Specification for Automotive Spark-Ignition Engine Fuel

    D4840 Guide for Sample Chain-of-Custody Procedures

    D4860 Test Method for Free Water and Particulate Contamination in Middle Distillate Fuels (Clear and Bright Numerical Rating)

    D4870 Test Method for Determination of Total Sediment in Residual Fuels

    D4952 Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test)

    D5304 Test Method for Assessing Middle Distillate Fuel Storage Stability by Oxygen Overpressure

    D5452 Test Method for Particulate Contamination in Aviation Fuels by Laboratory Filtration

    D6217 Test Method for Particulate Contamination in Middle Distillate Fuels by Laboratory Filtration

    D6227 Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component

    D6426 Test Method for Determining Filterability of Middle Distillate Fuel Oils

    D6751 Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels

    D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid FuelsFiltration and Culture Procedures

    D7463 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water

    D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

    D7687 Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

    D7978 Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated WaterThixotropic Gel Culture Method

    D8070 Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay

    E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods

    E1259 Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390C

    E1326 Guide for Evaluating Non-culture Microbiological Tests

    Other Standards

    98/8/EC Biocidal Products Directive Official Journal of the European Communities, 24.4.98, L123/163(1998).

    Test Method 2540D Total Suspended Solids Dried at 103105C Available from American Public Health Association, 800 I Street, NW Washington, DC 20001.

    TPC Publication No. 3

    Energy Institute Standards


    IP472 Determination of fungal fragment content of fuels boiling below 390C

    ICS Code

    ICS Number Code 27.060.10 (Liquid and solid fuel burners); 43.060.40 (Fuel systems)

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/D6469-20

    Citation Format

    ASTM D6469-20, Standard Guide for Microbial Contamination in Fuels and Fuel Systems, ASTM International, West Conshohocken, PA, 2020, www.astm.org

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