Journal Published Online: 01 March 1992
Volume 37, Issue 2

Isoelectric Focusing of Human Hair Keratins: Correlation with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Patterns and Effect of Cosmetic Treatments

CODEN: JFSCAS

Abstract

A new isoelectric focusing (IEF) technique in polyacrylamide gels with 6M urea and 1.5% Nonidet P40 has been developed to characterize human hair samples. The phenotypes demonstrated with this procedure has been correlated with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns described by other authors. The method described can be applied in the forensic science analysis of a single human hair. Using the same IEF technique we have studied the changes in electrophoretic patterns of cosmetically treated hair. The characteristics of the modifications observed and its utility in forensic science work are also discussed in this paper.

Author Information

Rodriguez-Calvo, MS
University of Santiago de Compostela, Galicia, Spain
Carracedo, A
University of Santiago de Compostela, Galicia, Spain
Muñoz, I
University of Santiago de Compostela, Galicia, Spain
Concheiro, L
University of Santiago de Compostela, Galicia, Spain
Pages: 7
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Stock #: JFS13251J
ISSN: 0022-1198
DOI: 10.1520/JFS13251J