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Significance and Use
5.1 This practice provides laboratory procedures for rating the relative bioresistance of metalworking fluid formulations, for determining the need for microbicide addition prior to or during fluid use in metalworking systems and for evaluating microbicide performance. General considerations for microbicide selection are provided in Practice E2169.
5.2 The factors affecting challenge population numbers, taxonomic diversity, physiological state, inoculation frequency and biodeterioration effects in recirculating metalworking fluid systems are varied and only partially understood. Consequently, the results of tests completed in accordance with this practice should be used only to compare the relative performance of products or microbicide treatments included in a test series. Results should not be construed as predicting actual field performance.
1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Methods D3946 and E686.
1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change, physical property change or some combination thereof.
1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant components.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D888 Test Methods for Dissolved Oxygen in Water
D1067 Test Methods for Acidity or Alkalinity of Water
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D3342 Test Method for Dispersion Stability of New (Unused) Rolling Oil Dispersions in Water
D3519 Test Method for Foam in Aqueous Media (Blender Test)
D3601 Test Method for Foam In Aqueous Media (Bottle Test)
D4627 Test Method for Iron Chip Corrosion for Water-Miscible Metalworking Fluids
D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
E70 Test Method for pH of Aqueous Solutions With the Glass Electrode
E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
E2169 Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids
E2523 Terminology for Metalworking Fluids and Operations
E2563 Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
E2564 Practice for Enumeration of Mycobacteria in Metalworking Fluids by Direct Microscopic Counting (DMC) Method
E2657 Test Method for Determination of Endotoxin Concentrations in Water-Miscible Metalworking Fluids
E2694 Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
E2889 Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
ICS Number Code 75.100 (Lubricants, industrial oils and related products)
UNSPSC Code 77121608(Pesticides pollution assessment); 10191500(Pesticides or pest repellents)