ASTM E2275 - 14

    Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance

    Active Standard ASTM E2275 | Developed by Subcommittee: E35.15

    Book of Standards Volume: 11.05


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    Significance and Use

    5.1 This practice provides laboratory procedures for rating the relative bioresistance of metalworking fluid formulations, for determining the need for microbicide addition prior to or during fluid use in metalworking systems and for evaluating microbicide performance. General considerations for microbicide selection are provided in Practice E2169.

    5.2 The factors affecting challenge population numbers, taxonomic diversity, physiological state, inoculation frequency and biodeterioration effects in recirculating metalworking fluid systems are varied and only partially understood. Consequently, the results of tests completed in accordance with this practice should be used only to compare the relative performance of products or microbicide treatments included in a test series. Results should not be construed as predicting actual field performance.

    1. Scope

    1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Methods D3946 and E686.

    1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change, physical property change or some combination thereof.

    1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant components.

    1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D888 Test Methods for Dissolved Oxygen in Water

    D1067 Test Methods for Acidity or Alkalinity of Water

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D3342 Test Method for Dispersion Stability of New (Unused) Rolling Oil Dispersions in Water

    D3519 Test Method for Foam in Aqueous Media (Blender Test)

    D3601 Test Method for Foam In Aqueous Media (Bottle Test)

    D4627 Test Method for Iron Chip Corrosion for Water-Miscible Metalworking Fluids

    D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

    E70 Test Method for pH of Aqueous Solutions With the Glass Electrode

    E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria

    E2169 Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids

    E2523 Terminology for Metalworking Fluids and Operations

    E2563 Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

    E2564 Practice for Enumeration of Mycobacteria in Metalworking Fluids by Direct Microscopic Counting (DMC) Method

    E2657 Test Method for Determination of Endotoxin Concentrations in Water-Miscible Metalworking Fluids

    E2694 Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

    E2756 Terminology Relating to Antimicrobial and Antiviral Agents

    E2889 Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment


    ICS Code

    ICS Number Code 75.100 (Lubricants, industrial oils and related products)

    UNSPSC Code

    UNSPSC Code 77121608(Pesticides pollution assessment); 10191500(Pesticides or pest repellents)


    DOI: 10.1520/E2275-14

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