This practice provides laboratory procedures for rating the relative bioresistance of metalworking fluid formulations, for determining the need for microbicide addition prior to or during fluid use in metalworking systems and for evaluating microbicide performance. General considerations for microbicide selection are provided in Practice E 2169.

The factors affecting challenge population numbers, taxonomic diversity, physiological state, inoculation frequency and biodeterioration effects in recirculating metalworking fluid systems are varied and only partially understood. Consequently, the results of tests completed in accordance with this practice should be used only to compare the relative performance of products or microbicide treatments included in a test series. Results should not be construed as predicting actual field performance.

1. Scope

1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Methods D 3946 and E 686.

1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change, physical property change or some combination thereof.

1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant components.

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D888 Test Methods for Dissolved Oxygen in Water

D1067 Test Methods for Acidity or Alkalinity of Water

D1193 Specification for Reagent Water

D3342 Test Method for Dispersion Stability of New (Unused) Rolling Oil Dispersions in Water

D3519 Test Method for Foam in Aqueous Media (Blender Test)

D3601 Test Method for Foam In Aqueous Media (Bottle Test)

D4012 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

D4627 Test Method for Iron Chip Corrosion for Water-Dilutable Metalworking Fluids

D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

E70 Test Method for pH of Aqueous Solutions With the Glass Electrode

E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria

E2169 Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids

Keywords

antimicrobial; bacteria; bactericide; biocide; bioresistant; coolant; fungi; fungicide; metalworking fluid; microbicide; mold; pesticide; yeast; Metalworking fluids/related media; Microbicides; Microorganisms; Pesticides; Yeasts; Antimicrobial formulations/testing; Bacteria/bacterial control; Biological data analysis; Bioresistance; Fungal influence/resistance;

ICS Code

ICS Number Code 75.100 (Lubricants, industrial oils and related products)

Citing ASTM Standards

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