Volume 15, Issue 1 (January 1987)

    Cytotoxicity of Incinerator Fly Ash on Alveolar Macrophages

    (Received 2 December 1985; accepted 18 June 1986)


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    Fly ash samples were collected from the electrostatic precipitator of a municipal incinerator in Hong Kong. The physical and chemical properties of fly ash particles of a diameter smaller than 10 μm were studied by a scanning electron microscope incorporated with an X-ray energy dispersive microanalyzer, atomic absorption spectrophotometer, and X-ray powder diffractometer. The particles were very heterogeneous in size and shape and had high levels of trace metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn).

    The cytotoxicity of fly ash particles was assessed using an in vitro culture assay of rat alveolar macrophages. It was found that the particles damaged the surface morphology of the cell, decreased the cell viability and phagocytic activity, and increased the release of cytoplasmic (lactate dehydrogenase) and lysosomal (β-glucuronidase) marker enzymes into the culture medium. No hemolytic effect could be measured on the rat red blood cells, indicating that the plasma membrane is not the target site of the incinerator fly ash.

    Author Information:

    Liu, WK
    The Chinese University of Hong Kong, Shatin, N.T., Hong Kong,

    Wong, MH
    Hong Kong Baptist College, Hong Kong,

    Tam, NFY
    Hong Kong Polytechnic, Hong Kong,

    Stock #: JTE11545J

    ISSN: 0090-3973

    DOI: 10.1520/JTE11545J

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    Title Cytotoxicity of Incinerator Fly Ash on Alveolar Macrophages
    Symposium , 0000-00-00
    Committee D34