Extraction and Analysis of Human Nuclear and Mitochondrial DNA from Electron Beam Irradiated Envelopes

    Volume 48, Issue 6 (November 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 November 2003

    Page Count: 7


    (Received 25 June 2003; accepted 21 June 2003)

    Abstract

    The United States Postal Service is considering methods such as electron beam irradiation to neutralize biological agents sent through the mail. While this is proven to reduce/eliminate pathogenic organisms, it may also degrade human genomic DNA and therefore hinder the ability to garner forensically informative genetic profiles. To determine the effects of electron beam irradiation on DNA typing, 16 white, standard letter-sized envelopes were licked. Half of the envelopes served as nonirradiated controls while the other half underwent irradiation at dosages sufficient to kill anthrax spores (29.3 and 51.6 kGy). Total cellular DNA was extracted from all envelopes; nuclear short tandem repeat loci, as well as the hypervariable region I from mitochondrial DNA, were amplified by means of the polymerase chain reaction. Short tandem repeat profiles and mitochondrial DNA sequence haplotypes were acquired on an ABI Prism® 310 Genetic Analyzer platform. Analysis of data from irradiated samples revealed evidence of DNA degradation; however, the ability to construct full genetic profiles from both nuclear and mitochondrial DNA remained largely unaffected. The use of the polymerase chain reaction, coupled with florescent fragment analysis and mitochondrial DNA sequencing, should be considered to profile biological material from evidence enduring irradiation to inactivate infectious agents.


    Paper ID: JFS2003109

    DOI: 10.1520/JFS2003109

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    Title Extraction and Analysis of Human Nuclear and Mitochondrial DNA from Electron Beam Irradiated Envelopes
    Symposium , 0000-00-00
    Committee E30