Recovery of Genomic Dna from Archived PCR Product Mixes for Subsequent Multiplex Amplification and Typing of Additional Loci: Forensic Significance for Older Unsolved Criminal Cases

    Volume 47, Issue 4 (July 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 2002

    Page Count: 11


    Burns, DM
    Griffith University, School of Biomolecular and Biomedical Science, Faculty of Science, Nathan Campus, Brisbane, QLD.

    Cox, KJ
    Forensic Biology Laboratory, Queensland Health Scientific Services, Brisbane, QLD.

    Patchett, KL
    Forensic Biology Laboratory, Queensland Health Scientific Services, Brisbane, QLD.

    (Received 4 January 2002; accepted 21 December 2001)

    Abstract

    A method for genomic DNA recovery from different types of PCR product mixes suitable for multiplex amplification and typing using the Profiler Plus™ STR typing system has been investigated. The application of this method is of significance in cases where the original DNA samples have been exhausted due to repeated typing analyses in an effort to maximize their evidentiary value. Such cases typically involve samples analyzed using the available DNA typing systems of the time which gave a markedly lower power of discrimination, either alone or in combination, compared to that of modern multiplex STR typing systems. It was found that an effective method for recovering genomic DNA from HLADQA1 _PM and CTT triplex amplification mixes, suitable for reproducible achievement of the complete Profiler Plus™ profile, involved the use of Amicon Microcon-100 microconcentrators. Interestingly, this method was not required to achieve the complete nine STR profile using D1S80 amplification mixes.


    Paper ID: JFS15444J

    DOI: 10.1520/JFS15444J

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    Title Recovery of Genomic Dna from Archived PCR Product Mixes for Subsequent Multiplex Amplification and Typing of Additional Loci: Forensic Significance for Older Unsolved Criminal Cases
    Symposium , 0000-00-00
    Committee E30