ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

A New Triplex STR System Without Irregular Alleles by Silver Staining and Its Potential Application to Forensic Analysis
Armour, JAL
Reader, School of Clinical Laboratory Sciences, University of Nottingham, Queen's Medical Centre,

Tamaki, K
Professor, Sapporo Medical University School of Medicine,

Mizutani, M
Research student, associate professor, assistant professor, postgraduate students, and professor, Postgraduate School of Medicine, Nagoya University,

Tanaka, M
Research student, associate professor, assistant professor, postgraduate students, and professor, Postgraduate School of Medicine, Nagoya University,

Uchihi, R
Research student, associate professor, assistant professor, postgraduate students, and professor, Postgraduate School of Medicine, Nagoya University,

Yamamoto, T
Research student, associate professor, assistant professor, postgraduate students, and professor, Postgraduate School of Medicine, Nagoya University,

Yoshimoto, T
Research student, associate professor, assistant professor, postgraduate students, and professor, Postgraduate School of Medicine, Nagoya University,

Huang, X-L
Postdoctoral fellow, Tokai University School of Medicine,

Katsumata, Y
Research student, associate professor, assistant professor, postgraduate students, and professor, Postgraduate School of Medicine, Nagoya University,

(Received 8 October 1999; accepted 22 June 2000)

Abstract

In order to increase the discriminating power of DNA analysis in forensic science, we devised a new triplex STR system using three novel STR loci we previously reported, D14S299 (wg1c5), D15S233 (wg1d1), and 9q2h2. We designated this system a CDH triplex system. The CDH triplex system showed a high discriminating power, especially in Caucasians. This system is composed of three STR loci showing only regular tetranucleotide repeat alleles. We easily enlarged the databases mainly of Japanese, using this system, and compared them with those of Caucasian and Chinese. This CDH triplex system therefore appears to be useful for forensic practice.

Keywords:
forensic science, DNA typing, short tandem repeat, population genetics, triplex, polymerase chain reaction, D14S299, D15S233, 9q2h2

Paper ID: JFS14995J
DOI: 10.1520/JFS14995J
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