Volume 41, Issue 1 (January 1996)

    Using Multiplex PCR Amplification and Typing Kits for the Analysis of DNA Evidence in a Serial Killer Case

    (Received 2 March 1995; accepted 16 May 1995)

    CODEN: JFSOAD

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    Abstract

    Analysis of DNA evidence in a serial killer case was performed using the AmpliType® HLA-DQ alpha-, AmpliType® PM-, and the GenePrint™ STR Multiplex System PCR Amplification Kits. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. DNA profiles from a single hair with attached sheath material, recovered from underneath the seat cover of the suspect's car seat were compared with DNA profiles derived from reference head hairs from a homicide victim. From the evidentiary sample only 9 ng of human DNA could be recovered. In a sample, where the quantity of DNA becomes a critical issue a powerful route is the simultaneous amplification of several loci (multiplex PCR). This is the first report where commercially available multiplex PCR amplification and typing kits have been introduced for the analysis of DNA evidence in a serial killer case and the analysis has been admitted in court.


    Author Information:

    Eisenberg, A
    Director, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX

    Budowle, B
    Research Chemist, Forensic Science Research and Training Center, Laboratory Division, Federal Bureau of Investigation Academy, Quantico, VA

    Hochmeister, MN
    Research Scientists, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne,

    Dirnhofer, R
    Director, Institute of Legal Medicine, University of Berne,

    Borer, UV
    Research Scientists, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne,


    Stock #: JFS13916J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13916J

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    Title Using Multiplex PCR Amplification and Typing Kits for the Analysis of DNA Evidence in a Serial Killer Case
    Symposium , 0000-00-00
    Committee E30