Work Item
ASTM WK86561

New Guide for Non-Animal Acute Toxicity Testing of Water-Dilutable Metalworking Fluids

1. Scope

1.1 This guide describes non-animal acute animal toxicity tests and sets forth the references for procedures to assess the acute toxicity of water-dilutable metalworking fluids as manufactured using non-live animal toxicity testing.
1.2 Although water-dilutable metalworking fluids are typically used at high dilution, dilution rates vary widely. Additionally, there is potential for exposure to the metalworking fluid as manufactured.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


metalworking fluids; acute toxicity testing; non-live animal toxicity testing; in vitro toxicity testing


E1302-2023, Guide for Acute Animal Toxicity Testing of Water-Miscible Metalworking Fluids, describes procedures and provides references for live-animal acute toxicity testing of metalworking fluids. Over the last twenty years, live animal (in vivo) toxicity testing has been supplanted with non-live animal toxicity testing methods and procedures. The new Guide will describe those new non-live animal methods and procedures now in use to evaluate metalworking fluids. Once approved, the new Guide will allow both producers and users of metalworking fluids to evaluate the acute toxicity characteristics using non-live animal methods.

The title and scope are in draft form and are under development within this ASTM Committee.


Developed by Subcommittee: E34.50

Committee: E34

Staff Manager: Travis Murdock

Work Item Status

Date Initiated: 05-25-2023

Technical Contact: John Howell

Item: 000