Work Item
ASTM WK76468

New Practice for DNA Extraction in Environmental Water Samples

1. Scope

This Practice discusses DNA extraction of environmental samples from environmental waters. It also discusses pre-processing of samples, removal of inhibitors, cleanup procedures, quality assurance procedures, data qualifiers, and corrective actions.

Keywords

Dehalococcoides; groundwater; bioremediation; monitored natural attenuation (MNA)

Rationale

Users of molecular biological tools need a standard practice for DNA extraction so that they know that the results provided by one laboratory are comparable to those developed by another laboratory. Consistency in procedures and results will foster greater usage of molecular tools.

The title and scope are in draft form and are under development within this ASTM Committee.

Details

Developed by Subcommittee: E50.04

Committee: E50

Staff Manager: Molly Lynyak

Work Item Status

Date Initiated: 04-07-2021

Technical Contact: Stephanie Fiorenza

Item: 000

Ballot:

Status: