Work Item
ASTM WK64471

New Test Method for Assessment of Fungal Biomass on Surfaces or Coatings by Measuring Fungal -N-Acetylhexosaminidase (NAHA) Activity

1. Scope

The promotion of knowledge, the development of test methods, practices, guides, and terminology pertaining to sampling and analysis of atmospheres, interpretation of data, the standardization of recognized and practiced methods for measurement of atmospheric quality, and sponsoring of discussions among those active in the study of air quality.
The work of the Committee will be coordinated with other ASTM Committees and other organizations having mutual interest.


fungal growth; fungal biomass; building materials; microbiological assay; mold; enzyme activity; -N-acetylhexosaminidase; NAHA; fluorometer; fluorescent count


This is an objective quantitative method for measuring total fungal biomass on surfaces and has a high reproducibility.

The title and scope are in draft form and are under development within this ASTM Committee.


Developed by Subcommittee: D22.08

Committee: D22

Staff Manager: Ashley Wiand

Work Item Status

Date Initiated: 07-20-2018

Technical Contact: Michael Kaniuga

Item: 001

Ballot: D22.08 (20-01)

Status: Will Reballot Item

Item: 003

Ballot: D22.08 (20-02)

Status: Will Reballot Item

Item: 002

Ballot: D22.08 (21-01)

Status: Negative Votes Need Resolution