Work Item
ASTM WK80644

New Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Porous and Non-porous Materials Against Staining by Streptomyces species (A Pink Stain Organism)

1. Scope

The purview of Committee E35 is the development of standard definitions, classifications, appropriate test methods, and recommended practices relating to efficacy, safety, quality, and impact in appropriate environments of pesticides, antiseptic and antimicrobial agents, biological agents, devices, and equipment.
These activities will be coordinated with the interests of related Committees in ASTM, and with other professional and governmental organizations.


antimicrobial efficacy; pink stain; plastics; polymeric non-solids; Streptomyces


When certain bacteria and mold species grow on the surface of non-rigid, flexible or “plasticized” polymers, metabolites such as pigments in the case of certain bacteria and melanin (dark stains from fungal growth) cause undesirable stains on the polymer surface. These stains may persist even after the surface growth is removed. This test method is used to determine the performance of antimicrobial agents used in or on synthetic polymeric porous and non-porous materials against staining by the Actinomycete, Streptomyces species. This organism has been chosen as an indicator organism, although other organisms have been known to cause undesirable staining in polymeric porous and non-porous materials.

The title and scope are in draft form and are under development within this ASTM Committee.


Developed by Subcommittee: E35.15

Committee: E35

Staff Manager: Brian Milewski

Work Item Status

Date Initiated: 01-24-2022

Technical Contact: Leon Estell

Item: 002

Ballot: E35.15 (22-01)

Status: Item Going To Next Ballot Level