Work Item
ASTM WK72506

New Guide for The Application of Molecular Biological Tools for Bioremediation: Natural and Enhanced Attenuation

1. Scope

This proposed scope is to establish an ASTM Standard Guide to develop a consistent approach for Molecular Biological Tools sampling, analysis (e.g., DNA/RNA extraction, PCR/qPCR/RT-qPCR, DNA/RNA sequencing), data processing (e.g., QA/QC, bioinformatics), and data interpretation to increase successful use of bioremediation approaches.


Biodegradation, MNA, Natural Attenuation


While analyses are widely available and some organizations have developed general guidance documents (ITRC 2011 & 2013, SERDP 2011 & 2014), consensus standardized approaches and methods that establish uniform guidelines, recommendations, considerations, criteria, and application principals have not been developed for end users (e.g., consultants, laboratories, or contaminated site managers). In the absence of a standard, environmental practitioners have adopted inconsistent applications of these tools which can lead to varying results across spatial and timescales, as well as from one site to another. Contaminated sites are largely growing in cost and complexity, and development and implementation of successful bioremediation mitigation strategies is dependent on consistent MBT data to assess, design, and monitor performance. The development of consensus standard documents through ASTM is paramount in meeting the needs of the remediation industry within the United States and elsewhere around the globe. The complexity and diversity of application of MBTs for bioremediation are not outlined in existing ASTM standards. The principal users of this standard will be industry project managers, regulators, consultants, laboratories, and contaminate site community stakeholders

The title and scope are in draft form and are under development within this ASTM Committee.


Developed by Subcommittee: E50.04

Committee: E50

Staff Manager: Molly Lynyak

Work Item Status

Date Initiated: 04-07-2020

Technical Contact: Stephanie Fiorenza

Item: 003

Ballot: E50 (22-05)

Status: Ballot Item Approved