Work Item
ASTM WK60554

New Test Method for Detection of Nitric Oxide Production

1. Scope

1.1This test method provides a protocol for rapid and quantitative measure of nitrite (NO2) a stable oxidative end-product of the antimicrobial effector molecule nitric oxide in cell culture medium. 1.2Nitric oxide (NO) has a critical role in several pathological conditions. 1.3This test method uses murine macrophage cell line RAW 264.7 as an in vitro model.


Nitric oxide; nanoparticulate; macrophages; immune response


None Provided

The title and scope are in draft form and are under development within this ASTM Committee.


Developed by Subcommittee: E56.08

Committee: E56

Staff Manager: Frank McConnell

Work Item Status

Date Initiated: 09-18-2017

Technical Contact: Tariq Fahmi

Item: 001

Ballot: E56 (22-02)

Status: Ballot Item Approved