SYMPOSIA PAPER Published: 01 January 2009
STP48749S

Performance of an Enzyme Electrode Designed for a Sulfide Monitoring Biosensor

Source

The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1 ′ dimeth-ylferrocene, catalyzes the oxidation of sulfide to sulfur.Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCI buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0–4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 uA. The sensitivity of the electrodes tested ranged from 0.2–0.3 μA mM−1.

Author Information

Sooknah, Reeta
Natural Resources Canada, Ottawa, Ontario, Canada
Papavinasam, Sankara
Natural Resources Canada, Ottawa, Ontario, Canada
Attard, Michael
Natural Resources Canada, Ottawa, Ontario, Canada
Revie, R.
Natural Resources Canada, Ottawa, Ontario, Canada
Gould, W.
Natural Resources Canada, Ottawa, Ontario, Canada
Dinardo, Orlando
Natural Resources Canada, Ottawa, Ontario, Canada
Price: $25.00
Contact Sales
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Developed by Committee: G01
Pages: 226–239
DOI: 10.1520/STP48749S
ISBN-EB: 978-0-8031-6997-5
ISBN-13: 978-0-8031-5522-0