SYMPOSIA PAPER Published: 01 January 2006
STP37697S

Development of an In Situ Biodegradation Technology by Using Anaerobic Micro-Organisms for Sediment Contaminated with Dioxins

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In this paper, an in situ bioremediation method is proposed for dioxin contaminated sediments and its efficiency is assessed in laboratory tests including microbial analysis. The main outcomes of this research are (1) Proposal of an in situ bioremediation method using anaerobic micro-organisms monitored by molecular microbial techniques. (2) It was shown that the proposed method can reduce dioxin concentrations and can increase populations of the dehalogenating micro-organism (Dehalococcoides sp.). (3) It was further shown that the dioxin degradation processes can be monitored using polymerase chain reaction (PCR) denaturing gradient gel electrophoresis and real-time PCR. Microbial analysis using molecular techniques was useful for the monitoring and management of the sediment remediation process.

Author Information

Hata, T
Research Center for Water Environment Technology, The University of Tokyo
Kurisu, F
Research Center for Water Environment Technology, The University of Tokyo
Yagi, O
Research Center for Water Environment Technology, The University of Tokyo
Mori, H
Materials and Geotechnical Engineering Research Group, Public Works Research Institute
Kuwano, R
Materials and Geotechnical Engineering Research Group, Public Works Research Institute
Kohashi, H
Material and Geotechnical Engineering Research Group, Public Works Research Institute
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Details
Developed by Committee: D18
Pages: 287–295
DOI: 10.1520/STP37697S
ISBN-EB: 978-0-8031-5512-1
ISBN-13: 978-0-8031-3408-9