SYMPOSIA PAPER Published: 01 January 1985

Synchronous-Excitation Fluorescence Applied to Characterization of Phenolic Species


Synchronous-excitation fluorescence (SEF) spectroscopy promises to be a quick, sensitive, and highly selective technique for the determination of hazardous contaminants. This work concentrates on detection of the phenols that pervade many areas of everyday life. Phenols frequently found in streams and groundwater as industrial pollutants also result as degradation products of green plants. The primary concern for these phenols is their known carcinogenic nature. In conjunction with derivative spectroscopy, synchronous-excitation fluorescence detects phenols in the parts per billion range and discriminates between common variants. By scanning the excitation and emission monochromotors with a 3-nm offset between them, each phenol species is characterized by a single peak. The water-Raman band, the prime interference at dilute concentrations in normal fluorescence, is also eliminated with the synchronous technique. Further, when derivatization is applied to the data, isomers, whose synchronous peaks occur within 5 nm of one another, can be separated from a mixture.

Author Information

Purcell, FJ
SPEX Industries, Inc., Edison, NJ
Kaminski, R
SPEX Industries, Inc., Edison, NJ
Obenauf, RH
SPEX Industries, Inc., Edison, NJ
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Developed by Committee: E13
Pages: 81–94
DOI: 10.1520/STP32773S
ISBN-EB: 978-0-8031-4928-1
ISBN-13: 978-0-8031-0412-9