SYMPOSIA PAPER Published: 01 January 1985

Metal Ion Sensors Based on Immobilized Fluorogenic Ligands


A metal ion sensor may be prepared by immobilizing a fluorogenic ligand on the common end of a bifurcated fiber optic. Fluorescence is excited through one arm of the fiber optic and observed through the other. When the immobilized ligand is placed in a solution of metal ion, some of the metal associates with the ligand causing its fluorescence characteristics to change. Equations are derived for the response of a sensor for systems where a 1:1 complex is formed.

We have successfully immobilized several ligands including morin, quercetin, and calcein to cellulose using cyanuric chloride as a coupling reagent. Immobilized morin is only very weakly fluorescent but forms fluorescent complexes with Al+3 and Be+2 and can be used to sense these ions. Immobilized calcein is fluorescent by itself but is quenched by several metal ions. We have also immobilized hydroxy napthol blue and 8-hydroxyquinoline-5-sulfonate on anion exchangers. In addition 2, 2′, 4-trihydroxy-azobenzene and p-tosyl-8-aminoquinoline have been immobilized to silica gel.

Author Information

Seitz, WR
University of New Hampshire, Durham, NH
Saari, LA
Instrumentation Laboratory, Inc., Lexington, MA
Zhujun, Z
Shaansi Normal University, Sian, Shaansi, People's Republic of China
Pokornicki, S
C. R. Bard Corp., North Reading, MA
Hudson, RD
Texaco, Inc., Beacon, NY
Sieber, SC
Yale University Medical School, New Haven, CT
Ditzler, MA
College of the Holy Cross, Worcester, MA
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Developed by Committee: E13
Pages: 63–77
DOI: 10.1520/STP32772S
ISBN-EB: 978-0-8031-4928-1
ISBN-13: 978-0-8031-0412-9