SYMPOSIA PAPER Published: 01 January 1984

Uniform Methods for Exposure Regimes in Aquatic Toxicology Experiments with Chemically Dispersed Oils


Many different approaches have been used recently in aquatic toxicity research for preparing and analyzing test solutions and dispersions of hydrocarbons. dispersants, hydrocarbon/dispersant mixtures, and oiled sediment mixtures. This variety is a result of the novelty of this research field. Therefore much of the published work is difficult to compare, summarize, and interpret. Both uniform and unique methods will contribute to a better understanding of the toxicology and potential field effects of chemically dispersed oils in marine waters. This paper summarizes some current methods and recommends adoption of certain principles and uniform methods for water and sediment studies. Specific items discussed are (1) choice of oils and dispersants; (2) degree of “weathering” of the experimental oils; (3) preparation of water-soluble fractions and oil dispersions in water; (4) choice of dispersant/oil ratios, especially considering dispersant effectiveness; (5) reagent mixing and premixing; (6) dosing of sediments; (7) chemical analysis of dispersed hydrocarbons; and (8) choice of exposure concentrations, composition, and durations. This paper is intended to encourage more uniformity in test conditions. This need for uniformity has been recognized internationally for several years.

Author Information

Wells, PG
Anderson, JW
Mackay, D
Price: $25.00
Contact Sales
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Developed by Committee: F20
Pages: 23–37
DOI: 10.1520/STP30226S
ISBN-EB: 978-0-8031-4906-9
ISBN-13: 978-0-8031-0400-6