SYMPOSIA PAPER Published: 01 January 1991

Tissue Culture as a Method for Evaluating the Biotransformation of Xenobiotics by Plants


Suspension cultures of Rosa cultivar (cv.) Paul's Scarlet were used as a model system to examine the metabolism of 1,3-dinitrobenzene (DNB), an industrial waste compound. In a 3-day period, 90% of the DNB supplied (96 nmol) was metabolized by approximately 12 g (fresh weight) of cells. The primary end product of DNB metabolism partitioned into the insoluble residue fraction of the extracted cells. This study demonstrates the usefulness of plant tissue cultures in evaluating the chemical influence of plants on xenobiotics.

Author Information

Wickliff, C
U.S. Environmental Protection Agency, Environmental Research Laboratory, Corvallis, OR
Fletcher, JS
University of Oklahoma, Norman, OK
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Developed by Committee: E47
Pages: 250–257
DOI: 10.1520/STP19519S
ISBN-EB: 978-0-8031-5173-4
ISBN-13: 978-0-8031-1422-7