SYMPOSIA PAPER Published: 01 January 1991

Characterization of Eight Soil Types Using the Bioassay


A recently developed bioassay protocol was used to characterize seven different soil types and Promix BX. The green alga Selenastrum capricornutum was used as the test organism. A ruggedness test which altered seven variables was conducted to identify procedural stipulations that should be carefully controlled in the Selenastrum capricornutum bioassay. Stimulatory or inhibitory effects as measured by population growth were compared to a control using two different nutritional methods termed proportional amendment (PA) and complete amendment (CA). Population growth, calculated effect (CE) values, and EC50 values are given for each soil. A wide range of effects were documented indicating a substantial difference among soils with respect to their ability to stimulate or inhibit algal population growth.

Author Information

Ireland, FA
Dixon Springs Agricultural Center, University of Illinois, Simpson, IL
Judy, BM
University of Missouri, Columbia, MO
Lower, WR
Environmental Trace Substances Research Center, University of Missouri, Columbia, MO
Thomas, MW
American Cyanamid Company, Birmingham, AL
Krause, GF
University of Missouri, Columbia, MO
Asfaw, A
University of Missouri, Columbia, MO
Sutton, WW
Environmental Research Laboratory, U.S. Environmental Protection Agency, Athens, GA
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Developed by Committee: E47
Pages: 217–229
DOI: 10.1520/STP19516S
ISBN-EB: 978-0-8031-5173-4
ISBN-13: 978-0-8031-1422-7