SYMPOSIA PAPER Published: 01 January 1990

Characterization of Xenobiotic Uptake Utilizing an Isolated Root Uptake Test (IRUT) and a Whole Plant Uptake Test (WPUT)


An isolated root uptake test (IRUT) and a whole plant uptake test (WPUT) were used to characterize the uptake and bioaccumulation of naphthol in two varieties of fescue (Festuca arundinaceae Schreb. var. KY-31 and var. Auburn) and one variety of clover (Trifolium pratense L. var. Redland). Whole plant uptake tests (WPUT) run for 24, 48, and 72 h revealed that the amount of radiolabel translocated to the shoots of both species was directly related to the length of exposure time. 14C-naphthol concentration in the roots of whole plants was directly proportional to naphthol concentration in solution between 0.1 and 0.3 μM. The transpiration stream concentration factor (TSCF) in both varieties of fescue decreased with increasing naphthol concentration in solution. 14C-naphthol elution from the apparent free space (AFS) was very rapid, occurring in less than 1 min. The AFS volume of clover and KY-31 fescue was determined to be approximately 10% of the total root volume. Isolated root tissue, incubated in 10 μM naphthol for 24 h, contained less than 1% of the radiolabel as recoverable parent compound.

Author Information

Krstich, MA
University of Tennessee, Knoxville, TN
Schwarz, OJ
University of Tennessee, Knoxville, TN
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Developed by Committee: E47
Pages: 87–96
DOI: 10.1520/STP19055S
ISBN-EB: 978-0-8031-5148-2
ISBN-13: 978-0-8031-1397-8