SYMPOSIA PAPER Published: 01 January 1991
STP17618S

Evaluation of a Method to Detect and in Water

Source

The current study outlines a technique to simultaneously detect Giardia and Cryptosporidium in raw and tap water samples. The influence of various procedural steps, including filtration, elution, flotation, filter mounting and microscopy, was determined for the method. Overall, the method recovered 48% to 69% of the Giardia and 25% to 42% of the Cryptosporidium in spiked water samples. The method had an averaged detection limit of 1 organism/100 L of tap water. Use of phase-contrast and Nomarski differential interference contrast microscopy could detect false-positive algal cells and determine cysts and oocysts having “non-viable type” morphologies.

Author Information

LeChevallier, MW
Norton, WD
Lee, RG
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Details
Developed by Committee: D19
Pages: 483–498
DOI: 10.1520/STP17618S
ISBN-EB: 978-0-8031-5163-5
ISBN-13: 978-0-8031-1407-4