A new method to evaluate the cut resistance of protective glove materials has been developed. The method consists of sliding a straight blade on a sample material and determining the horizontal blade displacement required to cut the material at a given load. A special arrangement allows a load applied on the sample material to be kept constant throughout the test. The effect on the cutting results of a) degradation in blade sharpness, b) blade speed, c) sample holder geometry (semi-circular or flat), and d) the load applied was characterized. The results demonstrated that a) the blade edge degraded even with soft materials such as neoprene; b) the blade speed had a negligible effect on the cutting results; c) the holder's geometry does not have a significant effect on the variability in the cutting results, but the use of a semi-circular holder is recommended; and d) the applied load is a function of the material's cut-resistance. It was found that the blade displacement increases non-linearly with the applied load. The cutting test conditions were set as follows: the blade is 70 mm long and its speed is 150 mm/min; the blade edge must be used only once. To evaluate the resistance of a material to cutting, tests must be performed with at least two different loads. The load required to cut a material to a standardized distance, namely 10 mm, is calculated by interpolating the experimental values. However, in the preferred method, the cut test is performed with three or four different loads and the load required to cut to 10 mm is calculated by a non-linear regression. Results are reproducible with a coefficient of variation (CV) lower than 16% with homogeneous materials. However, higher CV's are obtained with knitted materials such as Kevlar gloves, or steel reinforced materials.