The influences of test method and sample manipulation on toxicity of sediment and sediment pore water were evaluated using a series of toxicity tests with MicrotoxR and Hyalella azteca. Although the chemistry and toxicity of pore water extracted with vacuum-operated syringe and centrifuge were similar, the advantages of the vacuum-operated extractor outweighed those of the centrifuge. Sample disturbance and storage time influenced toxicity, but freezing, refrigeration, and pore-water volume did not. The inherent variability in assessment of toxicity from moderately contaminated sediments was adequately described by using 5–10 replicates per treatment. Feeding did not improve survival in control or reference treatments during 10-day toxicity tests. Static and static-renewal tests on pore water and solid-phase sediment were similar in sensitivity to assessing sediment quality.
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