SYMPOSIA PAPER Published: 01 January 1993

Correlation with Fetax of a Cellular Bioassay -Cell Health Assay of Water Quality -CHAWQ


We used albino Xenopus laevis embryos to test the correlation of a short-term, cellular, multiple-endpoint assay (Cell Health Assay of Water Quality- CHAWQ) with a 96 hour assay FETAX (Frog Embryo Teratogenicity Assay: Xenopus). We describe the hardware and instrumentation to perform the assay. We also describe the endpoints and methods to measure them. Exemplary endpoint measurements are presented: 6-Aminonicotinamide produced a decrease in the membrane potential of the embryo as measured by dye fluorescence. A potato glycoalkaloid, α-chaconine, increased dye fluorescence due to intracellular pH. Measurements of DNA using fluorescent dyes showed a normal increase in DNA until gastrulation. A clastogen — triethylenemelamine- was detected with hex-bisbenzamide dye. These data suggest that the CHAWQ assay may contain endpoints that predict acute or chronic macroscopic effects on an organism.

Author Information

Blankemeyer, JT
Oklahoma State University, Stillwater, OK
Stringer, BK
Oklahoma State University, Stillwater, OK
Bantle, JA
Oklahoma State University, Stillwater, OK
Friedman, M
USDA-ARS, Albany, CA
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Developed by Committee: E47
Pages: 146–158
DOI: 10.1520/STP13151S
ISBN-EB: 978-0-8031-5257-1
ISBN-13: 978-0-8031-1485-2