Although numerous studies have demonstrated the sensitivity of behavioral endpoints and the ecological relevance of behavioral toxicity, behavioral toxicity tests, including avoidance-preference tests, have been generally excluded from the hazard assessment process. One major obstacle to common acceptance of avoidance-preference responses is the lack of test standardization, which is not surprising given the numerous methods and taxa to consider and the relatively short tenure of research for any given area. Lack of standardized methodology, however, makes it difficult or impossible to compare results from different studies. Broad generic methodology is needed for standardized avoidance-preference tests that are appropriate for numerous species and exposure conditions. Our proposed standardized methodology includes a multiple-choice fluviarium, with video-based data acquisition and associated experimental protocols and statistical analyses. We believe the system should have extensive application as part of a standardized methodology for screening waterborne chemicals in toxicological avoidance-preference studies.