SYMPOSIA PAPER Published: 01 January 1998

Comparison of LC50 Results from Commonly Used Computer Programs


Using 50 data sets, this paper compares LC50s computed by several, commonly used computer programs. Programs evaluated include: Stephan, TOXSTAT®, ToxCalc™, U.S. Environmental Protection Agency (PROBIT and TSK), and this author's SAS® program. Numeric comparisons are made of both the LC50 estimate and its 95% confidence limits. An evaluation was made of the robustness of the programs to unusual data sets, the ease of use, and the clarity with which the programs provide results or error messages. The SAS LC50 program provided estimates for the greatest number of data sets. The probit LC50 point estimates provided by the EPA program and the SAS LC50 program were in the greatest agreement. TOXSTAT had the greatest disagreement with the other programs for the probit confidence limits. There was more agreement among the TSK estimates than among the probit estimates. Both EPA programs and the Stephan program require manual data entry with no ability to store the data. If a Windows® operating system environment is desired, either the SAS LC50 or the ToxCalc program is useful. Since SAS LC50 runs in the SAS environment and ToxCalc runs in the Excel™ environment, each is easier to use if one is already familiar with the respective environment.

Author Information

Sebaugh, JL
Sebaugh's Information Services, Lake Ozark, MO
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Developed by Committee: E47
Pages: 383–397
DOI: 10.1520/STP12177S
ISBN-EB: 978-0-8031-5384-4
ISBN-13: 978-0-8031-1485-2