SYMPOSIA PAPER Published: 01 January 1998

Comparison of Two Reference Toxicants and Their Effect upon the Growth and Development of in Axenic Culture


A static, 14 day laboratory bioassay using axenic Myriophyllum sibiricum Komarov was developed and utilized to determine the effects of two reference toxicants [zinc chloride (ZnCl2) and phenol] upon the morphological and physiological development of this species. For ZnCl2 the highest “no observable effect concentration” (NOEC) was 4.26 mg/L for root length. Based upon IC50 values, the most sensitive endpoint parameter for ZnCl2 was root length (range = 8.2–17.8 mg/L), followed by fresh weight (range = 13.2 – 32.4 mg/L) and root number (range = 13.3 – 38.6 mg/L). For phenol, the NOEC was 11.76 mg/L for three endpoint parameters (root number, total root length and fresh weight). Based upon IC50 values for phenol, the most sensitive endpoint parameter was root length (32.6 – 40.0 mg/L), followed by root number (45.8 – 57.0 mg/L) and fresh weight (48.2 – 75.9 mg/L). For both reference toxicants, the results were repeatable over time. As tested in this axenic culturing system, the ZnCl2 was slightly more toxic to the M. sibiricum than the phenol.

Author Information

Roshon, RD
University of Guelph, Guelph, ON
Stephenson, GR
University of Guelph, Guelph, ON
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Developed by Committee: E47
Pages: 200–214
DOI: 10.1520/STP12164S
ISBN-EB: 978-0-8031-5384-4
ISBN-13: 978-0-8031-1485-2