SYMPOSIA PAPER Published: 01 January 1998
STP12157S

Identification of Cytochrome P4501A Inducers in Complex Mixtures of Polycyclic Aromatic Hydrocarbons

Source

An in vitro ethoxyresorufin O-deethylase (EROD) assay was used to study the ability of individual polycyclic aromatic hydrocarbons (PAHs) and mixtures of PAHs to induce Ah receptor (AhR) mediated cytochrome P4501A activity in PLHC-1 fish hepatoma cells. The purpose was to identify the most potent inducers from a set of thirteen separate PAHs and describe interactions occurring in complex mixtures of these PAHs. Where possible, potency was expressed in terms of 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TCDD-EQ) by normalizing the PAH results to a TCDD standard curve. The most potent inducers were benzo(k)fluoranthene > benzo(a) pyrene ≈ benzo(b)fluoranthene > chrysene ≈ benzo(a)anthracene. At equal concentrations, these PAHs yielded potencies of 1670, 940, 655, 255, and 185 pg TCDD-EQ/g, respectively. Analysis of various mixtures of the thirteen PAHs suggested that complex interactions may be occurring.

Author Information

Villeneuve, DL
Michigan State University, Dept. of Zoology, Institute for Environmental Toxicology, and Pesticide Research Center, East Lansing, MI
De Vita, WM
University of Wisconsin-Stevens Point, College of Natural Resources, Stevens Point, WI
Crunkilton, RL
University of Wisconsin-Stevens Point, College of Natural Resources, Stevens Point, WI
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Details
Developed by Committee: E47
Pages: 92–104
DOI: 10.1520/STP12157S
ISBN-EB: 978-0-8031-5384-4
ISBN-13: 978-0-8031-1485-2