Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.
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