A two-level, three-variable full factorial experimental design was employed to probe the effects of temperature-modulated differential scanning calorimetry variables (heating rate, modulation frequency, and modulation amplitude) on the observed glass transitions of polybutadiene, poly(styrene-co-butadiene) and polystyrene. The statistical significance of individual variables was ascertained by Student's t-tests. Standard least-squares fitting of the model was employed to determine interaction effects between the variables. Frequency was determined to have a statistically significant effect on extrapolated onset glass transition temperature, half- height glass transition temperature, and the change in heat capacity across the glass transition for all three polymers. Within the confines of these experiments, changes in heating rate and amplitude did not have a statistically significant effect on glass transition measurement. No synergistic interaction effects were observed between the variables.