SYMPOSIA PAPER Published: 01 January 1988

Microbiological Contamination: Evaluation of Some Sampling and Laboratory Analytical Methods


The limitations and problems of representative sampling in naval fuel tanks have been investigated. Problems have been associated with access, with possibly atypical habitats (for example, entrained sounding tube or drain line samples), and with types of samplers available. In laboratory bottle scale trials for biocide efficacy, microbial interface growth has been quantified by biochemical determination of cellular protein following alkali extraction. This method is applicable to frozen stored samples. The significance of mixed microbial populations, in relation to coalescer impairment and to biocide treatment of the fuel, is also discussed.

Author Information

Hebda, AJ
Seatech Investigation Services, Ltd., Halifax, Nova Scotia, Canada
Morchat, RM
Defence Research Establishment Atlantic, Halifax, Nova Scotia, Canada
Brown, RG
Dalhousie University, Halifax, Nova Scotia, Canada
Jones, GM
Seatech Investigation Services, Ltd., Halifax, Nova Scotia, Canada
Price: $25.00
Contact Sales
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Developed by Committee: D02
Pages: 27–36
DOI: 10.1520/STP10039S
ISBN-EB: 978-0-8031-5026-3
ISBN-13: 978-0-8031-1186-8