We investigated the distributions of haplotypes for four Y-STR loci, named as DYS434, DYS438, DYS439, and A10, and developed a multiplex amplified for these Y-STR markers. A total of 100 samples were analyzed. Each sample was amplified utilizing multiplex PCR for four Y-STR loci. The PCR products were analyzed by non-denaturing polyacrylamide gel electrophoresis and visualized by silver staining. A total of 37 different haplotypes were found, and 16 haplotypes were being unique. Gene diversity ranged from 0.3945 at DYS434 to 0.7051 at DYS439. The haplotype diversity, chance of exclusion and discrimination power of four Y-STR loci was 0.9628, SE 0.00429. The results indicated that they were suitable Y linked markers for forensic applications in our population studied.
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