All 193 tested individuals have been involved in legal proceedings concerning various forensic testing. Buccal swabs have been taken as the DNA source and Chelex procedure was used for DNA extraction (1). The PowerPlex 16 kit (Promega Corp., Madison, WI) has been used to simultaneously amplify by PCR 15 STR loci. The STR loci are: D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA. Similar amounts of DNA have been used in all PCR reactions. Amplification was carried out as described previously (2).
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