Journal Published Online: 27 July 2005
Volume 50, Issue 5

Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electrophoresis with Short-End Injection



A simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 μm I.D. × 360μm O.D. with an effective separation length of 9 cm. The system was run at 60°C with an applied voltage of −8 kilovolts. Injection of each sample was for 1 sec at −50 mbar. UV detection was employed with the wavelength set at 195 nm. The background electrolyte consisted of 65 mM borate, pH 12.0. Samples and standards were prepared in the run buffer containing 2 mg/mL of mannose as an internal standard. Under these conditions a test mixture containing glucose, sucrose, lactose, mannitol and mannose as an internal standard was resolved within 5 min. The method was used to determine the concentration of carbohydrates in heroin seizure samples and synthetic heroin samples. The results were in good agreement with the reported values.

Author Information

Anastos, N
Deakin University, Geelong, VIC, Australia
Barnett, NW
Deakin University, Geelong, VIC, Australia
Lewis, SW
Deakin University, Geelong, VIC, Australia
Pearson, JR
Victoria Police Forensic Services Centre, Macleod, VIC, Australia
Kirkbride, KP
Forensic Science South Australia, Forensic Science Centre, Adelaide, SA, Australia
Pages: 5
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Stock #: JFS2004549
ISSN: 0022-1198
DOI: 10.1520/JFS2004549