Journal Published Online: 06 April 2005
Volume 50, Issue 3

Analysis of Fiber Dyes By Liquid Chromatography Mass Spectrometry (LC-MS) with Electrospray Ionization: Discriminating Between Dyes with Indistinguishable UV-Visible Absorption Spectra



Liquid chromatography mass spectrometry (LC-MS) is shown to provide high discriminating power for the identification of textile dyes that can not be reliably distinguished on the basis of their UV/visible absorption profile. Seven pairs of commercial dyes having nearly identical UV/visible absorption profiles and absorption maxima within 5 nm, were identified successfully by LC-MS. Two pairs of cotton fibers, which were indistinguishable by microspectrophotometry, were differentiated by LC-MS. A single wavelength UV/visible detector was used to monitoring dye elution from the column. The utility of the method for comparing questioned and known fibers is discussed.

Author Information

Huang, M
National Center for Forensic Science, University of Central Florida, Orlando, FL
Russo, R
University of Central Florida, Orlando, FL
Fookes, BG
University of Central Florida, Orlando, FL
Sigman, ME
National Center for Forensic Science and Department of Chemistry, University of Central Florida, Orlando, FL
Pages: 9
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Stock #: JFS2004223
ISSN: 0022-1198
DOI: 10.1520/JFS2004223