Journal Published Online: 06 April 2005
Volume 50, Issue 3

Multiplex PCR Development of Y-chromosomal Biallelic Polymorphisms for Forensic Application

CODEN: JFSCAS

Abstract

Single-nucleotide polymorphisms of Y chromosome (Y-SNPs) are a class of markers of interest in forensic investigations, because many of them show regional specificity, providing useful information about the geographic origin of a subject or evidence under investigation. A first multiplex with 7 SNPs (M35, M89, M9, M170, M172, M45, M173), which occur in the basal branches of the phylogenetic tree and are able to assign a subject to known most frequent European haplogroups, was designed. SNP genotyping was accomplished by hot-start PCR with primers amplifying fragments between 96 and 136 nucleotides, minisequencing, and capillary electrophoresis of extension products. Ninety seven subjects of known geographic provenance were studied, of which 68 from Europe. Of these, 57 had mutations found more frequently in European haplogroups and 11 more frequent in Asian populations. Subjects from non-European countries were also examined and had haplogroups common in their regions of provenance. Experiments with low molecular weight DNA gave positive amplification from 1 ng of DNA for all seven SNPs.

Author Information

Alessandrini, F
Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy
Turchi, C
Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy
Onofri, V
Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy
Buscemi, L
Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy
Pesaresi, M
Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy
Tagliabracci, A
Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy
Pages: 7
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Stock #: JFS2004147
ISSN: 0022-1198
DOI: 10.1520/JFS2004147