Journal Published Online: 01 January 2003
Volume 48, Issue 1

Allele Frequencies for Six STR Loci (D5S818, D7S820, D8S1179, D13S317, D16S539, FGA) in a Population Sample of North Italy

CODEN: JFSCAS

Abstract

Whole blood samples were collected from 170 unrelated healthy individuals living in the North Italy. Genomic DNA was extracted using GenomicPrep Blood DNA Isolation Kit (Amersham-Pharmacia-Biotech, Milano, Italy) and its concentration was determined spectrophotometrically. Two to five ng of target DNA was amplified in singleplex for FGA and D8S1179, while duplex amplification was adopting for D13S317/D16S539 and D5S818/D7S820. PCR amplification conditions were the same for all six loci and the program of amplification were carried out in a GeneAmp® PCR System 9700 Thermal Cycler (PE-Biosystems, Foster City, CA). The amplified products were separated and detected using the A.L.F. express DNA sequencer (Pharmacia-Biotech, Uppsula, Sweden). Allele designation was established following the recommendations of the DNA commission of the ISFH (1). Statistical analysis was performed as previously reported (2) by a computer program made from the authors using Excel spread sheets.

Author Information

Turrina, S
University of Verona, Verona, Italy
De Leo, D
University of Verona, Verona, Italy
Pages: 2
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Stock #: JFS2002305
ISSN: 0022-1198
DOI: 10.1520/JFS2002305