Journal Published Online: 01 January 2003
Volume 48, Issue 1

Autosomal Microsatellite Profile of Three Socially Diverse Ethnic Tamil Populations of India

CODEN: JFSCAS

Abstract

Blood samples were obtained by venipuncture from random, consenting individuals of the three populations: Kongu Vellala Gounder (56), Irular (54), and Chakkiliyar (49). DNA was isolated using standard phenol/chloroform procedure (1). Fifteen autosomal microsatellite loci were amplified using the PowerPlex® 16 system (Promega Corporation, Madison) according to manufacturer's recommendations (2). The amplified products were separated by denaturing gel electrophoresis using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City, CA). Alleles were determined by comparison with the allelic ladder included in the kit.

Author Information

Sitalaximi, T
Trivedi, R
Central Forensic Science Laboratory, West Bengal, India
Kashyap, VK
Central Forensic Science Laboratory, West Bengal, India
Pages: 4
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS2002291
ISSN: 0022-1198
DOI: 10.1520/JFS2002291