Blood samples were obtained by venipuncture from random, consenting individuals of the three populations: Kongu Vellala Gounder (56), Irular (54), and Chakkiliyar (49). DNA was isolated using standard phenol/chloroform procedure (1). Fifteen autosomal microsatellite loci were amplified using the PowerPlex® 16 system (Promega Corporation, Madison) according to manufacturer's recommendations (2). The amplified products were separated by denaturing gel electrophoresis using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City, CA). Alleles were determined by comparison with the allelic ladder included in the kit.

Author Information

Sitalaximi, T
Trivedi, R
Central Forensic Science Laboratory, West Bengal, India
Kashyap, VK
Central Forensic Science Laboratory, West Bengal, India
Pages: 4
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Stock #: JFS2002291
ISSN: 0022-1198
DOI: 10.1520/JFS2002291