Blood samples were collected in tubes containing ACD from 121 unrelated individuals who asked paternity analysis in The Clinical Hospital of the University of Chile during the years 2000 and 2001. The method presented by Comey (1) was used to remove DNA from the samples. The three STR were amplified using 1 ng of DNA for each PCR reaction, and the fragments electrophoresed through 6% acrylamide gels and silver stained, according to the manufacturer's recommendations (2).

Author Information

Carriquiry, F
University of Los Andes, Chile
Bacallao, K
University of Chile, Chile
Acuna, M
University of Chile, Chile
Cifuentes, L
University of Chile, Chile
Pages: 1
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Stock #: JFS2002217
ISSN: 0022-1198
DOI: 10.1520/JFS2002217