Blood stains from 116 unrelated individuals from Angola (West Africa) were stored using bloodstain card and extracted with the standard Chelex method. PCR amplification was performed in a GeneAmp® PCR System 9600, using the AmpFℓSTR® Profiler Plus™ Amplification Kit, according to the manufacturer's recommendations. Electrophoresis of the PCR products was carried out in an ABI Prism™ 377 DNA Sequencer, using 5% denaturing Long Ranger® gels. Sample genotypes were determined with Genotyper® (version 2.0), by comparison with supplied allelic ladders and an internal size standard (GS-500Rox).
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