Journal Published Online: 01 January 2003
Volume 48, Issue 1

Angola Population Data with AmpFℓSTR™ Profiler Plus

CODEN: JFSCAS

Abstract

Blood stains from 116 unrelated individuals from Angola (West Africa) were stored using bloodstain card and extracted with the standard Chelex method. PCR amplification was performed in a GeneAmp® PCR System 9600, using the AmpFℓSTR® Profiler Plus™ Amplification Kit, according to the manufacturer's recommendations. Electrophoresis of the PCR products was carried out in an ABI Prism™ 377 DNA Sequencer, using 5% denaturing Long Ranger® gels. Sample genotypes were determined with Genotyper® (version 2.0), by comparison with supplied allelic ladders and an internal size standard (GS-500Rox).

Author Information

Geada, H
Faculdade de Medicina da Universidade de Lisboa Instituto Nacional de Medicina Legal-Delegação de Lisboa
Viriato, L
Instituto Nacional de Medicina Legal-Delegação de Lisboa
Vieira-Silva, C
Instituto Nacional de Medicina Legal-Delegação de Lisboa
Cruz, C
Instituto Nacional de Medicina Legal-Delegação de Lisboa
Ribeiro, T
Instituto Nacional de Medicina Legal-Delegação de Lisboa
Espinheira, R
Instituto Nacional de Medicina Legal-Delegação de Lisboa
Pages: 2
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Stock #: JFS2002202
ISSN: 0022-1198
DOI: 10.1520/JFS2002202