Whole blood samples were collected from healthy unrelated donors born and living in Aragon (n = 136) and Asturias (n = 120). DNA was isolated using standard phenol-cloroform extraction method. Amplification was carried out according to Nievas (1) in a PE 2400 thermocycler (PE Biosystems). Each locus was amplified individually, except DYS389I/II and DYS385 (two loci each with the same set of primers). The recommendations of the International Society for Forensic Genetics were followed for typing and interpretation (2). In addition, we successfully took part in the quality control exercise for Y-STRs organized by Dr. Lutz Roewer, Institute of Legal Medicine, Humboldt University, Berlin, Germany (3). Y-STR haplotype variability was evaluated by means of Analysis of Molecular Variance (AMOVA) (4,5).

Author Information

Martinez-Jarreta, B
University of Zaragoza, Zaragoza, Spain
Nievas, P
University of Zaragoza, Zaragoza, Spain
Abecia, E
University of Zaragoza, Zaragoza, Spain
Hinojal, R
University of Oviedo, Asturias, Spain
Budowle, B
FBI Academy, Quantico, VA
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Stock #: JFS2002201
ISSN: 0022-1198
DOI: 10.1520/JFS2002201