Journal Published Online: 01 January 2003
Volume 48, Issue 1

A Simple Method of DNA Extraction and STR Typing from Urine Samples Using a Commercially Available DNA/RNA Extraction Kit

CODEN: JFSCAS

Abstract

We devised a simple DNA extraction procedure suitable for STR typing of urine sample. Use of a commercially available DNA/RNA extraction kit equipped with a silica-gel-based membrane made it possible to omit the recovery of urinary nucleated cells by sedimentation before the extraction. Successful genotyping of the TH01, HumTPO and multiplex STRs was achieved using aliquots of urine as small as 100 µL. Furthermore, application of this DNA extraction procedure to frozen urine samples provided STR allele results comparable to results obtained from fresh samples. Therefore, this extraction procedure is considered to be effective for STR typing of urine samples in both the frozen and aqueous state. Furthermore, addition of sodium azide to fresh urine samples prolonged their storage duration even at room temperature.

Author Information

Yasuda, T
Fukui Medical University, Fukui, Japan
Iida, R
Fukui Medical University, Japan
Takeshita, H
Gunma University School of Medicine, Gunma, Japan
Ueki, M
Fukui Medical University, Fukui, Japan
Nakajima, T
Gunma University School of Medicine, Gunma, Japan
Kaneko, Y
Gunma University School of Medicine, Gunma, Japan
Mogi, K
Gunma University School of Medicine, Gunma, Japan
Tsukahara, T
Criminal Investigation Laboratory, Fukui, Japan
Kishi, K
Gunma University School of Medicine, Gunma, Japan
Pages: 3
Price: $25.00
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Details
Stock #: JFS2002184
ISSN: 0022-1198
DOI: 10.1520/JFS2002184