Journal Published Online: 01 January 2003
Volume 48, Issue 1

A Novel Fluorescent Quadruplex STR Typing System and the Allele Frequency Distributions in a Thai Population



We have previously reported a new triplex amplification and typing system by silver staining for three short tandem repeat (STR) loci, 9q2h2 (D2S3020), D15S233, and D14S299 without "microvariant" alleles such as .1, .2, and, .3 alleles in the Japanese population. In the present study, we established a new quadruplex system with an additional locus D7S809 using primer sets labeled with fluorescent multi-color dyes. Using this system, we genotyped 183 Thai people, found only one “microvariant” allele (allele 20.2) at D7S809, and calculated allele frequencies and some statistical properties at these four STR loci. From these allele frequencies at four STR loci, we performed three statistical analyses including a homozygosity test, a likelihood ratio test, and an exact test for Hardy-Weinberg equilibrium (HWE). Deviations from HWE (p < 0.05) were observed only in the two tests at the locus D7S809.

Author Information

Yoshimoto, T
Nagoya University, Nagoya, Japan
Yamamoto, T
Nagoya University, Nagoya, Japan
Mizutani, M
Nagoya University, Nagoya, Japan
Uchihi, R
Nagoya University, Nagoya, Japan
Ohtaki, H
Nagoya University, Nagoya, Japan
Katsumata, Y
Nagoya University, Nagoya, Japan
Waiyawuth, W
Mahidol University, Bangkok, Thailand
Songsivilai, S
Mahidol University, Bangkok, Thailand
Pages: 6
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Stock #: JFS2002006
ISSN: 0022-1198
DOI: 10.1520/JFS2002006